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      我的位置:首頁(yè)  >  技術(shù)文章  >  ARBOR ASSAYS 免疫測(cè)試產(chǎn)品 試劑盒

      ARBOR ASSAYS 免疫測(cè)試產(chǎn)品 試劑盒

      更新時(shí)間:2024-08-01      瀏覽次數(shù):645

      ARBOR ASSAYS 免疫測(cè)試產(chǎn)品 試劑盒

       

      Arbor Assays公司成立于2007年,位于密歇根州的安娜堡,公司的三位主創(chuàng)始人均為有多年科研經(jīng)驗(yàn)的生命和臨床科學(xué)家。其宗旨是為臨床使用的重要生物分子建立高質(zhì)量的檢測(cè)和免疫測(cè)試產(chǎn)品。在生物領(lǐng)域建立健全的免疫檢測(cè)和酶活性試劑盒以定量生物分子。

      主要為臨床重要生物分子提供高質(zhì)量的檢測(cè)和免疫測(cè)試產(chǎn)品,如乙酰膽堿酯酶活性熒光檢測(cè)試劑盒、谷胱甘肽熒光檢測(cè)試劑盒、血紅蛋白比色檢測(cè)試劑盒等。種類多樣,操作簡(jiǎn)單,靈敏度高。

      認(rèn)識(shí)到對(duì)靈敏度、速度、便利性、質(zhì)量和服務(wù)的需求,我們研究、開(kāi)發(fā)和制造用于臨床重要生物分子的最高質(zhì)量的檢測(cè)和免疫測(cè)定試劑盒。

      Arbor Assays公司的主要產(chǎn)品包括:

      DetectX® Kits

      Inhibitors & Activators

      AbX® Antibodies & Affinity Resins

      Detection Systems

      Components & Ancillary Reagents

      所需材料:

      類固醇標(biāo)準(zhǔn)品,用于檢測(cè)萃取效率

      ACS級(jí)乙醇(或乙酸乙酯)

      玻璃試管

      處理流程:

      確保樣品wan全干燥,并對(duì)樣品進(jìn)行粉末化處理,以提高萃取回收率。如果可能的話清除任何大顆粒,如草屑。我們建議通過(guò)使用試劑盒的AB分析緩沖液及已知濃度的類固醇溶液檢測(cè)結(jié)果來(lái)確定提取效率,詳細(xì)信息請(qǐng)參考下面的萃取效率部分。乙醇或乙酸乙酯萃取樣品及萃取效率控制如下:

      1.稱取≥0.2 g干燥的糞便固體放入管中。樣品可以通過(guò)被動(dòng)干燥、溫和加熱(≤60°C)或冷凍的方式來(lái)進(jìn)行干燥處理。

      2.每0.1 g固體(0.1 g糞便固體/mL)加入1 mL乙醇(或乙酸乙酯)并密封。

      3.用力搖晃至少30分鐘。

      4.在4°C下,將樣品在5,000 rpm下離心15分鐘。取上清放入一個(gè)干凈的管中。如果密封得當(dāng),該樣本可在≤-20°C下保存至少一個(gè)月。

      注:含有低水平分析物的樣品可以通過(guò)干燥提取液和在少量分析緩沖液中重懸,來(lái)達(dá)到濃縮的效果。用乙酸乙酯提取的樣品在加入緩沖液之前,必須wan全干燥以去除溶劑。從步驟4中轉(zhuǎn)移測(cè)量體積(蒸發(fā)體積)的上清到一個(gè)干凈的管中,在離心蒸發(fā)裝置或氮?dú)庀抡舭l(fā)至干燥。干燥、提取的樣品可在干燥器中冷凍≤-20°C保存?;蛴靡掖?如100μL)溶解干燥提取的樣品,直接進(jìn)行步驟5。

       5.以上步驟為通用步驟,可根據(jù)需要量進(jìn)行等比例體積調(diào)整。將步驟4中提取的樣本上清液100μL加入最少400μL的AB緩沖液中(總重構(gòu)體積=100μL+400μL)。室溫下渦旋混勻,靜置5min,重復(fù)至少兩次渦旋和靜置步驟。對(duì)于免疫分析,測(cè)定中乙醇含量通常需要≤5%,需要根據(jù)所選擇的試劑盒中描述的最大乙醇含量耐受情況,用檢測(cè)緩沖液進(jìn)行稀釋。

      6.根據(jù)試劑盒說(shuō)明書(shū)對(duì)稀釋后的樣本進(jìn)行ELISA檢測(cè)。

       

      下面是ARBOR ASSAYS公司的部分熱銷產(chǎn)品

      產(chǎn)品名稱

      貨號(hào)

      規(guī)格

      Goat Anti-Mouse IgG Fc, 25MG

      A008-25MG

      25MG

      Cyclic AMP Direct ELISA Kit (1 Plate)

      K019-H1

      1x96 well plate

      Corticosterone ELISA Kit (1 Plate)

      K014-H1

      1x96 well plate

      Protein Kinase A (PKA) Activity Assay Kit (1 Plate)

      K027-H1

      1x96 well plate

      Aldosterone ELISA Kit (1 Plate)

      K052-H1

      1x96 well plate

      Urea Nitrogen (BUN) Detection Kit (2 Plate)

      K024-H1

      2x96 well plate

      Cortisol ELISA Kit (1 Strip plate)

      K003-H1

      1x96 well plate

      PGFM ELISA Kit (1 Plate)

      K022-H1

      1x96 well plate

      Triiodothyronine (T3) ELISA Kit (1 Plate)

      K056-H1

      1 Kit

      FRAP™ (Ferric Reducing Antioxidant Power) Detection Kit (2 Plate)

      K043-H1

      2x96 well plate

      Hemoglobin Detection Reagent, 20ML

      C038-20ML

      20ML

      Ceruloplasmin Colorimetric Activity Kit (2 Plate)

      K035-H1

      2x96 well plate

      ThioStar™ Fluorescent Thiol Substrate, 100UG

      L002-100UG

      100UG

      Cyclic GMP Direct Chemiluminescent ELISA Kit (1 Plate)

      K020-C1

      1x96 well plate

      Progesterone ELISA Kit (1 Plate)

      K025-H1

      1x96 well plate

      Testosterone ELISA Kit (1 Plate)

      K032-H1

      1x96 well plate

      Estrone-3-Glucuronide (E1G) ELISA Kit (1 Plate)

      K036-H1

      1x96 well plate

      Thyroxine (T4) ELISA Kit (1 Plate)

      K050-H1

      1x96 well plate

      Prostaglandin E2 (PGE2) Multi-Format ELISA Kit (1 Plate)

      K051-H1

      1x96 well plate

      Tranylcypromine

      X042-1EA

      1EA

      Triiodothyronine (T3) ELISA Kit (5 Plate)

      K056-H5

      5x96 well plate

      Glutathione Colorimetric Detection Kit (4 Plate)

      K006-H1

      4x96 well plate

      Cyclic GMP Direct ELISA Kit (1 Plate)

      K020-H1

      1x96 well plate

      Progesterone ELISA Kit (5 Plate)

      K025-H5

      5x96 well plate

      Cyclic AMP Direct ELISA Kit (5 Plate)

      K019-H5

      5x96 well plate

      Estradiol ELISA Kit (1 Plate)

      K030-H1

      1x96 well plate

      Oxytocin ELISA Kit (1 Plate)

      K048-H1

      1x96 well plate

      Estrone-3-Glucuronide (E1G) ELISA Kit (5 Plate)

      K036-H5

      5x96 well plate

      Pregnanediol-3-Glucuronide (PDG) ELISA Kit (5 Plate)

      K037-H5

      5x96 well plate

      Hemoglobin Standard, 300UL

      C037-300UL

      300UL

      DetectX® Estrone-3-Sulfate (E1S) Antibody, 3ML

      C133-3ML

      3ML

      Creatinine Urinary Detection Kit (2 Plate)

      K002-H1

      2x96 well plate

      Butyrylcholinesterase Fluorescent Activity Kit (2 Plate)

      K016-F1

      2x96 well plate

      Corticosterone ELISA Kit, (5 Plate)

      K014-H5

      5x96 well plate

      Cyclic GMP Direct Chemiluminescent ELISA Kit (5 Plate)

      K020-C5

      5x96 well plate

      Superoxide Dismutase (SOD) Colorimetric Activity Kit (2 Plate)

      K028-H1

      2x96 well plate

      Catalase Colorimetric Activity Kit (2 Plate)

      K033-H1

      2x96 well plate

      Glutathione Fluorescent Detection Kit (1 Plate)

      K006-F1

      1x96 well plate

      Pregnanediol-3-Glucuronide (PDG) Standard, 625UL

      C128-625UL

      625UL

      Thyroxine (T4) ELISA Kit (5 Plate)

      K050-H5

      5x96 well plate

      Acetylcholinesterase Fluorescent Activity Kit (2 Plate)

      K015-F1

      2x96 well plate

      Creatinine Serum Kit (2 Plate)

      KB02-H1

      2x96 well plate

      Hydrogen Peroxide Fluorescent Detection Kit (2 Plate)

      K034-F1

      2x96 well plate

      Creatinine Serum Low Sample Volume Kit (384-well Plate)

      KB02-H1D

      1x384 well plate

      Recombinant Obelin, 100UG

      L001-100UG

      100UG

      Dry DMSO, 20ML

      X022-20ML

      20ML

      Dry DMSO, 2ML

      X022-2ML

      2ML

      Urea Nitrogen Standard, 1ML

      C089-1ML

      1ML

      NADPH lyophilized, 1EA

      X045-1EA

      1EA

      DetectX® PGE2 Conjugate, 13ML

      C179-13ML

      13ML

      17-Hydroxyprogesterone ELISA Kits

      K053-H

      1 Kit

      Arg8-Vasopressin (AVP) ELISA Kits

      K049-H

      1 Kit

      Arg8-Vasopressin (AVP) Chemiluminescent ELISA Kits

      K049-C

      1 Kit

      Androstenedione ELISA Kits

      K070-H

      1 Kit

      Aldosterone Chemiluminescent ELISA Kits

      K052-C

      1 Kit

      Allopregnanolone ELISA Kits

      K061-H

      1 Kit

      Adrenocorticotropic Hormone (ACTH) ELISA Kits

      K072-H

      1 Kit

      Alkaline Phosphatase (ALP) Colorimetric Activity Kit

      K082-H1

      1 Kit

      3′,3′-Cyclic GAMP ELISA Kits

      K073-H

      1 Kit

      20-Hydroxyecdysone ELISA Kit

      K066-H

      1 Kit

      2′,3′-Cyclic GAMP STING-Based FRET Detection Kits

      K081-F

      1 Kit

      2′,3′-Cyclic GAMP ELISA Kits

      K067-H

      1 Kit

      Cyclic GMP Direct ELISA Kits – Improved Sensitivity

      K065-H

      1 Kit

      Endothelin-1 (ET-1) ELISA Kit

      K045-H1

      1 Kit

      Estrone-3-Sulfate (E1S) ELISA Kits

      K038-H

      1 Kit

      Assay Buffer, 5X

      X065-110ml

      110ml

      Blocking Buffer, 10X

      X109-25ml

      25ml

      Goat anti-Rabbit IgG Coated Plate

      X016-1EA


      HRP Conjugate Diluent

      X076-300ml

      300ml

      TMB Substrate

      X019-55ml

      55ml

      Mesotocin Solution (500ng/mL)

      X127-625UL


      5-Azacytidine

      P012-50MG

      50mg

       


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